FIGURE 6.

Overlap spectra of bovine serum albumin with (a) linagliptin, (b) rabeprazole sodium, and (c) their 1:1 formed complex. The enclosed area was formed between (A) bovine serum albumin fluorescence and (B) UV absorption spectra. The primary and secondary Y‐axes were expressed as fluorescence intensity and UV absorbance, respectively, while the X‐axis was denoted by the wavelength (nm). The energy transformation from the donor to the ligand can be confirmed by the sufficient overlap between the two curves. The curves of the fluorescence spectrum of the bovine serum albumin and absorption spectrum of the ligand overlaid that indicated the higher possibility of the existence of energy transfer between donor and acceptor molecules