FIG. 1.

(a) Hexon replacement strategy. A map of the genome of Av1LacZ4 is shown. The locations of the relevant restriction enzyme sites used for the manipulation of the genome are indicated. Arrows indicate the positions of the regions coding for the hexon and that for the β-galactosidase reporter gene. The locations of the L1, L2, and L4 loops within the hexon are shown. (b) Alignment of the complete Ad5 hexon amino acid sequence with that of the chimeric hexon of Av12LacZ (done with DNASTAR software). The replacement of the Ad5 sequence shown in panel a results in replacement of the Ad5 native sequence between amino acids 59 and 907. Identical amino acids are indicated by asterisks. Gaps in the sequence alignment are indicated by dashes. L1, L2, and L4 regions are shaded.