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. 2023 Apr 5;2(2):e102. doi: 10.1002/imt2.102

Figure 2.

Figure 2

Adrenocortical carcinoma (ACC) harbors intratumor microbes. (A) Comparison of two different sequencing strategies in intratumor microbe detection for NR microbiome data. The Bray–Curtis dissimilarity was used to assess the differences in microbial communities identified from different WGS or from WGS and RNA‐Seq. The box plot on the top and the density plot on the bottom shows the Bray–Curtis dissimilarity distribution. (B) Principal coordinate analysis (PCoA) for ACC and PCPG, based on the Bray–Curtis dissimilarity. Permutational multivariate analysis of variance (PERMANOVA) gave the p value. (C) Relative abundance of microbes in each microbiome data with different levels of filtering. Only the top five genera of each microbiome data were shown. (D) Representative images of fluorescence in situ hybridization (FISH) staining of 16S rRNA (up) and immunohistochemistry (IHC) staining of LPS (down) in tissue containing 37 ACC samples. (E) Representative images of comparison between positive result (up) and the negative control (down) in FISH staining of 16S rRNA (left) and IHC staining of LPS (right). Arrows were used as indication for positive signals.