Figure 2:

Ram deletion results in a reduction in CD8 T cells. (A) A number of CD4 and CD8 double negative (DN), double positive (DP) and single positive T cells in the thymus in control (Ram^fl/fl, n = 5) and Ram cKO (n = 5) mice. Representative FACS plot, right panel, including % in each gate. Number of CD4 and CD8 T cells in (B) spleen (control (n = 5) and Ram cKO (n = 4) mice), (C) peripheral lymph nodes (pLNs) (control (n = 5) and Ram cKO (n = 5) mice) and (D) mesenteric lymph nodes (mLNs) (control (n = 4) and Ram cKO (n = 5) mice). (E) Percentage of CD4 and CD8 CD62L high, CD62L/CD44 high and CD62L low T cells in spleen (top panel, control (n = 5) and Ram cKO (n = 5) mice), peripheral lymph nodes (pLNs, middle panel, control (n = 5) and Ram cKO (n = 5) mice), and mesenteric lymph nodes (mLNs, bottom panel, control (n = 5) and Ram cKO (n = 4) mice). Dots indicate biological replicates, lines indicate means, and P values from Student’s t test. Gating strategies are depicted in Supplementary Figs. 1 and 2