Fig. 1.

Schematic of two-component tetrahedral nanoparticle design using ProteinMPNN. The rotational (ω) and translational (r) symmetrical degrees of freedom of a set of previously described T33 docked configurations were sampled using the Rosetta SymDofMover to generate a range of closely related backbones. The backbones of two trimers (one A, one B) were extracted and new amino acid sequences at the A–B interface were generated with ProteinMPNN. The sequences were threaded onto the component backbones using the Rosetta PackRotamerMover to enable evaluation in the context of the full symmetric assembly using Rosetta scoring metrics.