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. 2024 Apr 3;10(14):eadl5012. doi: 10.1126/sciadv.adl5012

Fig. 4. ARFRP1 controls AP-1 localization to Golgi membranes and Golgi-to-lysosome export of SARS-CoV-2 Envelope.

Fig. 4.

(A) VeroE6 cells were transfected with a plasmid encoding E-mEmerald and stained with antibodies raised against endogenous AP1G1 and ARFRP1, or endogenous AP1G1 and TGN46. Images representative of 25 and 15 imaged cells, respectively. (B and C) WT or ARFRP1−/− VeroE6 cells were fixed and stained with antisera raised against ARFRP1 or AP1G1, and perinuclear localization of AP1G1 was scored in the accompanying quilt from 50 imaged cells (C). (D and E) Plasmids encoding the indicated ARFRP1 proteins were transfected into ARFRP1−/− VeroE6 cells. Cells were fixed and stained with antisera raised against ARFRP1 or AP1G1, and the perinuclear localization of AP1G1 was scored in the accompanying quilt from over 50 imaged cells per condition across three independent experiments (E). Transfected cells indicated by asterisks. (F) WT or ARFRP1−/− VeroE6 cells were transfected with a plasmid encoding E-mEmerald and stained with antibodies raised against endogenous AP1G1. Images representative of 14 or 23 imaged cells, respectively. In microscopy panels, scale bars are 10 μm.