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. 2024 Apr 3;10(14):eadl0389. doi: 10.1126/sciadv.adl0389

Fig. 6. Metabolic impact of Drp1 K38A in C2C12 murine myocytes and Drosophila.

Fig. 6.

(A) Dose-response of AAV-Drp1-DN on Drp1 protein level in C2C12 myotubes (n = 3 biological replicates). (B) Immunoblots of Sdhaf2 and Drp1 in the cytosolic and mitochondrial fractions of C2C12 myotubes treated with AAV-Drp1-DN [1600 viral particle (vp) per cell]. Densitometric quantification was performed by normalizing Sdhaf2 and Drp1 proteins to TFAM or actin (n = 3 biological replicates). (C) OCR of C2C12 myotubes treated with vehicle or AAV-Drp1-DN includes the following substrates pyruvate and malate or (D) succinate and rotenone (n = 8 biological replicates). (E) Succinate treatment elevates both Drp1 and Sdhaf2 protein in the mitochondrial fraction of C2C12 myotubes (n = 3 biological replicates). (F) Succinate elevates complex II containing respiratory supercomplexes (n = 3). (G) TCA cycle intermediates and related metabolites in control and siDrp1 Drosophila (n = 4 to 5). All values are presented as means ± SEM; *P < 0.05 determined by unpaired Student’s t test two-tailed.