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. 2024 Apr 3;12:RP88144. doi: 10.7554/eLife.88144

Figure 5. Functional FGFR1-mediated responses to different ligands.

(A) FGFR1 concentration in the plasma membrane of HEK 293T cells at t=2 min following ligand addition for FGF8, FGF9, and no ligand control. (B) HEK 293T cell viability after ligand exposure and 6 days of starvation for varying ligand concentrations. (C) Apoptosis of HEK 293T cells under starvation conditions, exposed to varying concentrations of FGF8 and FGF9. Results are summarized in Supplementary file 5. (D) RCSFgfr1 cells were treated with FGF4, FGF8, and FGF9 for 48 hr, and the levels of collagen type 2 were determined by western blot. Actin serves as a loading control. (E) Dose-response curves describing collagen type 2 loss. (F) Dose-response curves for growth arrest of RCSFgfr1 cells after 72 hr, in response to FGF4, FGF8, and FGF9. Data are from at least three biologically independent experiments.

Figure 5—source data 1. FGFR1 membrane concentration, cell viability, and apoptosis data plotted in Figure 5A, B, and C.
Figure 5—source data 2. Original files for the western blots in Figure 5D.
Figure 5—source data 3. PDF containing Figure 5D and original western blots.
Figure 5—source data 4. Collagen amounts, used to generate the dose-response curves for collagen type 2 loss in Figure 5E.
Figure 5—source data 5. Cell counts, used to generate the dose-response curves for growth arrest in Figure 5F.

Figure 5.

Figure 5—figure supplement 1. Total cellular expression of FGFR1 in the stable FGFR1 line.

Figure 5—figure supplement 1.

Shown are results for three different biologically independent samples in the absence of ligand, three samples that have been treated with FGF8 for 2 min, and three samples that have been treated with FGF9 for 2 min. All the expressions are the same.
Figure 5—figure supplement 1—source data 1. Original files for the western blots in Figure 5—figure supplement 1.
Figure 5—figure supplement 1—source data 2. PDF containing Figure 5—figure supplement 1 and original western blots.