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. 1998 Aug;72(8):6911–6916. doi: 10.1128/jvi.72.8.6911-6916.1998

FIG. 3.

FIG. 3

The cooperative transactivation activity of Tax requires the TAR element. (A) Schematic representation of the wild-type HIV-1 LTR and the mutants with various cis element deletions. The nucleotide acid number is based on the numbering of the complete sequence of pNL4-3 derived from GenBank. LTR272-773 flanks a full-length promoter, but the upstream cis sequences immediately before the USF element were deleted. LTR296-773 covers the complete promoter region with the upstream sequence deletion immediately after the USF site. LTR329-773 contains the entire promoter but the upstream region immediately before the duplicated κB sites was deleted. LTR1-536 covers the entire promoter and enhancer, but the cis elements immediately after the TAR sequence and HS4 region were deleted. LTR1-477 (LTRΔTAR) includes the complete upstream modulatory region and the core region but the TAR sequence was deleted. The wild-type LTR and all LTR mutants were linked to the SEAP gene. (B) The transfection of Jurkat TAg cells was performed using Tax (0.6 μg) and Tat (0.1 μg) as well as various LTR constructs (0.2 μg/each) as indicated in the figure. The data are representative of two independent experiments.