FIG. 4.
Tax’s transactivation to the HIV-1 LTR is κB element dependent. (A) Structures of wild-type LTR (LTRwt) and LTRΔκB. The wild-type HIV-1 LTR contains a complete upstream modulatory region as well as the core region and the TAR sequence. LTRΔκB was constructed by deleting all upstream modulatory region including the duplicated κB elements, but the core and the TAR regions are reserved. Both LTR fragments were linked to the SEAP gene. (B) Tax or TaxFS and Tat together with the wild-type LTR (LTRwt) or LTRΔκB reporter plasmid were transiently cotransfected into Jurkat TAg cells. The SEAP activity was quantified 48 h after transfection. The data shown below represent two independent experiments.