Fig. 1.

The overview of this study. 14 samples from the NCC cohort were analysed through STRT sequencing. By separately detecting differentially-expressed genes and key genes of TME landscape, EPCAM cells, T cells and Ma/Mo cells, 11 genes (HLA-DPB1, FAM83A, ITGB4, OAS1, FHL2, FSCN1, S100P, SFTPD, SPP1, DBH-AS1, CST3) were obtained to construct a prognostic and immunotherapeutic predictive signature with validation from public datasets and in-house cohorts. Abbreviations: STRT, Single-cell Tagged Reverse Transcription; EPCAM, epithelial cell adhesion molecule; Ma/Mo, macrophage and monocyte; TME, tumour microenvironment; HR, hazard ratio.