Fig. 2.

Method for isolation of GABA SVs. (a) Cartoon schematic (BioRender) of GABA SV isolation method. (b) Coomassie-stained SDS PAGE analysis of GABA SV isolation fractions including starting material LP2, flow through, and elution fractions 1–8. (c) Western blot analysis of GABA SV isolation fractions (same samples as in panel b). Top: Western blot using a rabbit polyclonal antibody against the luminal region of vGAT. Bottom: Western blot using a mouse polyclonal antibody against synaptophysin. Both proteins were co-blotted on the same membrane. (d) Western blots of mouse brain homogenate, LP2, flowthrough, and elution fractions. While all samples were loaded at equal volume, they were not loaded at equal protein amount or concentration. Antibody information is provided in Supplementary Table 1.