Fig. 3.

Electron microscopy imaging of GABA SVs. (a) Representative dynamic light scattering (DLS) measurement of purified GABA SVs plotted as a frequency distribution of measured particle diameters. n = 3 SV isolations, three DLS measurements. (b) Representative negative stain micrograph of purified GABA SVs. On the right, two magnified view examples of individual GABA SVs were taken from different representative micrographs. (c) Representative cryo-EM micrograph of isolated GABA SVs, with a physical pixel size of 0.243 nm/pixel. Cropped magnified views of individual GABA SVs from cryo-EM micrographs, with yellow arrows pointing to large visible protein densities, likely the v-ATPase. (d) Histogram displaying the frequency distribution of GABA SV diameters measured from cryo-EM micrographs using ImageJ; the dotted line represents the mean diameter value of 40.4 ± 0.5 nm. n = 2 isolations, 10 micrographs, 100 measured vesicles. All micrographs are available in the data repository associated with this work. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)