Fig. 4.

Association of GABA SVs with PM vesicles. (a) Cartoon schematic (BioRender) of the GABA SV-PM association assay. (b–e) Representative examples of individual association events, plotting baseline-normalized fluorescence vs. time, the red traces report the fluorescence due to the Alexa-647 dye present on the GABA SVs while green traces report the sulforhodamine B fluorescence inside the PM vesicles. See Supplementary Table 2 for details. (f) Bar graph detailing the relative distribution of association types observed in the total collected dataset of association movies. n = 3 technical replicates, 25 movies, 9728 p.m. vesicles, 320 association events. Number of Ca²⁺-independent fusion events was 3 out of 296 single vesicle association events. Shown are the mean and standard error of the mean, and the student's t-test was used to test statistical significance. All association data are available in the data repository associated with this work. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)