Fig. 6.

Effect of enasidenib treatment in the chondrogenic differentiation pathway and proliferative status. (a–c) Heatmap plots depicting the expression values of genes belonging to the GO BP chondrocyte differentiation pathway according to enasidenib treatment for each cell line. (b–c) Histological analysis of formalin-fixed paraffin-embedded T-CDS17#1 cell spheroids growth in chondrocyte differentiation medium with or without (control) 10 μM enasidenib for 21 days. Representative images of H&E and PAS-alcian staining (b) and quantification of PAS-alcian stain (c) are displayed. Scale bars = 100 μm. Error bars represent the standard deviation of three independent experiments. (d) Heatmap plot showing the expression values of those genes belonging to the mRNA expression signature developed by Nicolle et al. (2019), including their microarray experiments and the classification of chondrosarcoma samples into E1/E2 subtypes (n = 102). (e) Bubble plots showing the top 5 most significant pathways (ORA, FDR <0.05) from the MSigDB GO BP collection in each cell type for up-regulated (left) and down-regulated (right) E1/E2 genes.