Fig. 3.

The exported PfHSP40s reported to interact with human HSP70. Three-dimensional models of the J domains for the malarial proteins PFE0055c (PF3D7_0501100), KAHSP40 (PFB0090c/PF3D7_0201800), PFA0660w (PF3D7_0113700), and eCiJp (PF11_0034/PF3D7_1102200) are shown. The J domains of type II (green cartoons; PFE0055c, KAHSP40, and PFA0660w) and type IV (blue cartoon; eCiJp) PfHSP40s are shown. The conserved RK and HPD motifs are shown as gray sticks. The positive charge is shown on blue colored surface, the negative charge is shown on red colored surface, and neutral potentials are shown on white colored surface. The surface electrostatic potential was calculated by APBS. The PFA0660w J domain was obtained from the Protein Data Bank (PDB code 6RZY), and homology models were prepared for the other J domains using SWISS-MODEL (6RZY was the template for PFE0055c and KAHSP40; 4J7Z was the template for eCiJp).¹⁰⁸ The models were graphically rendered using PyMol 2.5.2 (PyMOL Molecular Graphics System, Version 2.0 Schrödinger, LLC). Abbreviations used: HSP, heat shock protein; PfHSP, Plasmodium falciparum HSP.