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. 2024 Apr 3;24(1):67. doi: 10.1007/s10238-024-01323-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Characterization of isolated sEVs. A Representative transmission electron microscopy images of serum-derived sEVs isolated using size exclusion chromatography (scale bar, 200 nm) from CRC patient (i) and healthy control samples (ii). B Representative graphs by DLS analysis indicating concentration and size distribution of isolated particles from CRC patient (i) and healthy control samples (ii). C Representative Western blot images showing enrichment of EV markers CD63, CD81, TSG101, and Alix with a low level of endoplasmic reticulum proteins (calnexin) in serum-derived sEVs