Table 1.

ATP content of glucose-deprived and glucose-fed astrocytes

	Incubation time (h)	ATP content (nmol/mg)	Extracellular LDH activity (%)	n
Untreated	0	30 ± 5	0	39
No glucose	24	8 ± 2***	8 ± 8	39
3 mM glucose	24	26 ± 4*	5 ± 5	23

Cultured primary astrocytes were incubated for 24 h in glucose- and amino acid-free incubation buffer in the absence (No glucose) or the presence of 3 mM glucose. Cellular ATP content and cellular LDH activity for untreated cells (before the incubation) and the cellular ATP content and the extracellular LDH activity (in % of the initial cellular LDH activity) after the 24 h incubations were determined. The initial cellular LDH activity at the onset of the incubation was 167 ± 43 nmol/(min × well). The data represent mean values ± SD obtained in 23 and 39 individual experiments performed on 17 and 30, respectively, independently prepared cultures. The significance of differences (ANOVA with Bonferroni post hoc test) compared with the data obtained for untreated cells is indicated by *p < 0.05 and ***p < 0.001