TABLE 3.
Transcriptional activation of lacZ by the interaction of NC protein and Ψ RNA
| RNA hybrida | Fusion proteina | Activationb |
|---|---|---|
| HIVΨ-MS2 | Gal4AD-HIV Gag | +++ |
| HIVΨ-MS2 | Gal4AD-HIV NC | +++ |
| HaMSVΨ-MS2 | Gal4AD-HIV NC | +++ |
| IRE-MS2 | Gal4AD-HIV NC | +++ |
| —c | Gal4AD-HIV NC | − |
a,b As in Table 1, except that the transformants were selected for uracil and leucine prototrophy for 4 days due to lower growth rate. Yeast colonies transformed only with pGal4AD-HIV NC were selected for leucine prototrophy.
—, no plasmid encoding an RNA hybrid was introduced.