Fig. 1.

PfAgo-targeted DNA cleavage mediated by a single gDNA. A A single gDNA can mediate PfAgo activity for 600-nucleotide (bp) long DNA targets to produce DNA cleavage products. B A single gDNA can assist PfAgo-mediated cleavage of 95-bp DNA targets, producing distinct banding patterns from reactions containing a pair of gDNAs. C A cleavage fragment with a 5′-phosphate end can function as a renewed gDNA that can initialize an additional round of DNA cleavage if the reverse complement sequence of the original target is present. D Schematic of single gDNA-assisted PfAgo-mediated activity for targeted DNA cleavage