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. 2021 Jun 11;8(1):46. doi: 10.1186/s40643-021-00401-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — RADAR for multiplex detection of nucleic acids. A Schematic of one-tube four-fluorophore multiplexing using orthogonal single-gDNA-assisted PfAgo-mediated cleavage activity, with each gDNA designed for respective ssDNA targets. B In-sample multiplexed detection of four ssDNA targets with corresponding gDNAs designed in an orthogonal manner. C Multiplexed detection of four HPV dsDNA targets with corresponding gDNAs designed in an orthogonal manner