Table 2.
PD-L1 expression in both large resection specimens (Cohort A) and small-sized liver biopsies (Cohort B) in patients with iCCA, after applying the scoring system for interpretation of the staining
| PD-L1 immunostaining | Cohort A | Cohort B | P value |
|---|---|---|---|
| Tumoral cells, n (%) | |||
| Negative | 10 (20.4) | 73 (83.9) | |
| Positive, mild | 5 (10.2) | 3 (3.44) | |
| Positive, moderate | 18 (36.73) | 9 (10.34) | < 0.001 |
| Positive, intense | 16 (32.65) | 2 (2.29) | |
| Tumor infiltrating immune cells | |||
| Negative | 19 (38.77) | 67 (77.01) | |
| Positive, mild | 2 (4.08) | 4 (4.59) | |
| Positive, moderate | 18 (36.73) | 10 (11.49) | |
| Positive, intense | 10 (20.4) | 2 (2.29) | < 0.001 |
| Not available* | 0 | 4 (4.59) | |
| Epithelial cells in the tumor invasion front | |||
| Negative | 40 (81.63) | 40 (45.97) | |
| Positive, mild | 5 (10.2) | 0 | |
| Positive, moderate | 2 (4.08) | 0 | |
| Positive, intense | 2 (4.08) | 0 | 0.009 |
| Not available* | 0 | 47 (54.02) | |
| Immune cells from the tumor invasion front | |||
| Negative | 40 (81.63) | 39 (44.82) | |
| Positive, mild | 2 (4.08) | 0 | |
| Positive, moderate | 1 (2.04) | 6 (6.89) | 0.632 |
| Positive, intense | 6 (12.24) | 0 | |
| Not available* | 0 | 42 (48.27) | |
*Data not available due to lack of benign tissue in some cases from Cohort B Negative = intensity grade *percentage of positive cells < 3 Positive = intensity grade * percentage of positive cells ≥ 3