Fig. 6.

MTT and Migration assay of MDA-MB-231 cells. The treatment of MDA-MB-231 cells with the conditioned medium of the different experimental groups of macrophages showed that after 24 h and 48 h, there was no significant difference between treatment groups. However, after 72 h, the conditioned medium of macrophages treated with miRNA-loaded exosomes decreased cell growth and proliferation of MDA-MB-231 cells in a IL-4-induced M2 macrophage and b TAM. Overexpression of miR-130 or miR-33 in exosomes inhibited the migration capacity of MDA-MB-231 cells in c IL-4-induced M2 macrophage and d TAM. MDA-MB-231 cells were cultured in the upper compartment of the transwell and then were placed in 24 well plate containing conditioned media from differently groups of treated macrophages. After 24 h, the number of migrated cells was counted manually *P < 0.05, **P < 0.01, ***P < 0.001