Fig. 4.
Assessment of prolonged protective antitumor immunity after radiotherapy (RT), anti-PD-L1, and anti-VEGF therapies. Mice were rechallenged with two-fold higher dose of Lewis lung carcinoma (LLC) cells (4 × 105 cells) on the opposite flank on day 40. The growth curve of 4 × 105 cells injected in the untreated mice was measured as reference. Tumor growth curves (a) and survival curves (b) in different treatment groups are plotted. Data represent means ± SEM measured on the indicated days. *p < 0.05 and **p < 0.01. Experimental groups contained five mice per group. Studies were conducted twice in two independent experiments with an interval of 1 month, and the presented data are the combined data of the two independent experiments