Fig. 6.
Evaluation of memory T cells in tumor-infiltrating lymphocytes (TILs) and splenocytes after radiotherapy (RT), anti-PD-L1, and anti-VEGF therapies. C57BL/6 mice were subcutaneously injected into the flank with 2 × 105 LLC cells. At 9 days after tumor implantation, the tumor-bearing mice were randomized into seven treatment groups: control, anti-VEGF (100 µg on day 0, 3, 6, and 9; total 400 µg), anti-PD-L1 (100 µg on day 1, 4, 7, and 10; total 400 µg), RT (40 Gy/4 fx on day 1, 2, 3, and 4), RT + anti-VEGF, RT + anti-PD-L1, and RT + anti-PD-L1 + anti-VEGF. Ten days after RT, infiltrating lymphocytes were isolated from the tumor microenvironments and spleen to obtain cell suspensions for surface staining. Quantitative data for the proportions of the various memory T cell populations in TILs (a) and in the spleen (c). Flow cytometric analysis of memory markers on CD8+ T cells isolated from TILs (b) and spleen (d). Data represent means ± SEM measured. **p < 0.01. Experimental groups contained five mice per group. Studies were conducted twice in two independent experiments with an interval of 1 month, and the presented quantitative histograms (a) and (c) are the combined data of the two independent experiments