Fig. 3.
Granulocytic, but not monocytic, myeloid-derived suppressor cells (MDSC) were decreased in tumor tissues of SV-fed LLC tumor-bearing mice. a Mice were fed control or SV food starting 10 days prior to tumor inoculation. C57BL/6 mice were subcutaneously challenged with 3 × 105 LLC cells. Mice were sacrificed on day 21. Tumor infiltrated leukocytes (TIL), splenocytes (SP) and bone marrow cells (BM) were isolated from tumor-bearing mice. G-MDSC (Ly6G+Ly6C+) and M-MDSC (Ly6G−Ly6C+) in TIL, SP and BM were analyzed by flow cytometry and presented as dot plots. b, c Statistical analysis of MDSC percentages in TIL (b) and Spleen (c) obtained from (a) is presented. *p < 0.05, ***p < 0.001, n = 5 mice per group. d The expression of MHC class II and Arginase type I of M-MDSC was analyzed by flow cytometry and presented as dot plots. e, f Statistical analysis of the percentage of MHCII + (e) and Arginase I + (f) in M-MDSC in TIL and spleen obtained from (d) is presented. n = 3 mice per group. Error bars indicate SEM. *p < 0.05, ANOVA