TABLE 1.
Abilities of laboratory-adapted and primary SIVmac251 to replicate in PBMC and CEMx174 cellsa
| Virus (host cell) | Day | Replicationb in:
|
||
|---|---|---|---|---|
| rPBMC | hPBMC | CEMx174 | ||
| Laboratory-adapted (H9) | 4 | <30 | <30 | 953 |
| 9 | <30 | 39 | >3,000 | |
| 11 | <30 | <30 | >30,000 | |
| Primary (H9) | 4 | 35 | 75 | 621 |
| 9 | >3,000 | 1,507 | >3,000 | |
| 11 | 10,979 | 1,980 | >30,000 | |
| Primary (rPBMC) | 4 | 313 | 297 | 2,088 |
| 9 | >3,000 | >3,000 | >3,000 | |
| 11 | 11,396 | 16,248 | >30,000 | |
| Primary (hPBMC) | 4 | >3,000 | 569 | >3,000 |
| 9 | >3,000 | >3,000 | >3,000 | |
| 11 | 13,261 | 19,609 | >30,000 | |
a
Stocks of laboratory-adapted and primary SIVmac251 were grown in the indicated host cells and were used to inoculate rhesus PBMC (rPBMC), human PBMC (hPBMC), and CEMx174 cells; an equal volume of virus was used for inoculation of each cell type.
b
Viral p27 concentrations in culture supernatants were quantified on days 4, 9, and 11 of incubation and are given in picograms per milliliter.