Fig. 5.
a Workflow of the in vivo experiment. NSG mice were subcutaneously inoculated in the flank with ARP-1 cells (5 × 106/mouse). When palpable tumors (> 5 mm) were detected, the mice were randomly divided into four groups (Control, Bor, Bor + MΦs, and Bor + IL-32γ-educated MΦs, n = 4 per group). MΦs were injected intratumorally only at the time of the first Bor administration. The Bor dosage was 2 μg/mouse/every 3 days. b Representative image of tumor volumes at the end point of the experiment. c Statistical results for the tumor weight and volume at the end point of the experiment. Data show the mean ± SEM of at least three mice per group. d Immunofluorescence analysis of CD138, Ki67 and cleaved caspase-3 expression in tumor tissues from mice in different groups. Scale bar, 50 μm. e Representative and summarized results for flow cytometry analysis of the number of CD11b+CD206+ M2 MΦs in tumor tissue. Data show the mean ± SEM of at least three mice per group. *p < 0.05