Fig. 2.

Cell-mediated immune responses induced by AMV002. a–b T cell responses to HPV16 E6 and E7 peptides were determined using PBMCs collected at baseline day 0 (D0) and at days 7, 35 and 63 after the first, second and third dose of AMV002. PBMCs were restimulated with two pools of E6 peptides or one pool of E7 peptides and cell numbers secreting IFNγ per 10^6 cells were determined by ELISpot. a Cumulated fold increase over pre-immunisation spot-forming cells (SFC) count for each subject at each time point for each peptide pool calculated as SFC (peptide/time point) − SFC (no peptide/time point)/SFC (peptide/pre-immunisation) − SFC (no peptide/pre-immunisation). b Number of SFC per 10⁶ mononuclear cells for each peptide pool at each time point for each study participant, corrected for “no peptide” SFC (SFC (peptide/time point) − SFC (no peptide/time point))