Fig. 3.

IBI322 selectively binds to dual antigen-expressing cells in the presence of RBCs. a Schematic of the experimental design to assay for selectivity in binding to H292 tumor cells (CD47/PD-L1 double positive cells) in the presence of excessive RBCs (CD47 single positive cells). H292 cells were labeled with CFSE and mixed with a 20-fold excess of RBCs. Cell mixtures were incubated with primary antibody prior to staining with APC anti-human Fc secondary antibody, then analyzed by flow cytometry. b, c Percentages of H292 tumor cells (CFSE +) and RBCs (CFSE −) within the antibody-bound (APC +) population