Fig. 2.

Characterization of anti-MSLN CAR-T cells. A Schematic representation of retroviral CAR constructs of the humanized scFv (G11), linked to the CD8α Signal peptide, intracellular signaling domains CD28, (G4S)3 Linker, and CD3ζ in tandem. B Proportion of MSLN virus-infected isolated human T cells determined by flow cytometry. T cells and mock CAR-T cells served as controls. C, D and E Cell phenotype of anti-MSLN CAR-modified T cells was assessed by flow cytometry. Twelve days after lentivirus CAR transduction, the subsets and phenotype of T cell, Mock CAR-T and MSLN CAR-T cells were analyzed by FACS, including the expression of CD4, CD8, CD62L, CD45RA, CCR7 and CD45RO