Fig. 2.

Identification of 16 clusters and immune cell types in “before” and “after” samples. a All 16 clusters were identified and shown with t-SNE plot. b T-SNE plot showing cell distribution in “before” and “after” Pembrolizumab treatment samples. c Identification of immune cell types using t-SNE. Using canonical cell markers: T cells-CD3D, CD4 (CD4+ T cells), CD8A (CD8+ T cells), LEF1 and CCR7 (Naïve T cells); NK cells-NKG7 and GZMB; B cells-CD20 (MS4A1), IGHM and CD79; Macrophages-CD68; Dendritic cells-CD1C; Erythrocytes-HBD, the 16 cell clusters were identified as naïve T cells, NK cells, NKT cells, B cells, Macrophages, Dendritic cells and Erythrocytes. d Relative percentage of immune cell types in blood samples (number of one cell type/number of all cell types) of before and after Pembrolizumab treatment