Fig. 2.

ADCC activities mediated by anti-CD99 mAb MT99/3. CFSE-labeled Z138, Granta-519, RC-K8, MM1R and L-363 cell lines (target cells) were co-cultured with a freshly isolated mouse splenocytes (effector cells) or b IL-2 activated mouse splenocytes (effector cells) at various E:T ratios in the presence of anti-CD99 mAb (mAb MT99/3) or isotype-match control (mIgG2a) or without Abs (No mAb) for 4 h at 37 °C. The dead cells were analyzed by flow cytometry. Percent cytotoxicity was calculated as [(Dead target cells (%) − spontaneous death (%))/(100 − spontaneous death)] × 100%. The experiments were carried out in triplicate. The values are shown as mean ± SEM. One-way ANOVA followed by Tukey's multiple comparisons test was used for comparison, *P < 0.05. **P < 0.01. ***P < 0.001. ****P < 0.0001. ns not statistically significant