Fig. 3.

CDC activities mediated by anti-CD99 mAb MT99/3 against B lymphoma cell lines. Z138, Granta-519, RC-K8, MM1R and L-363 cell lines were plated into RPMI 1640 medium supplemented with 5% rabbit serum or heat-inactivated rabbit serum (Heated serum). mAb MT99/3 or isotype-matched control (mIgG2a) were added or kept in culture medium (no mAb) and incubated for 2 h at 37 °C. MTT reagent was added then continuously incubated for 4 h. The formazan crystal was dissolved and the absorbance was measured at 570 nm. Each experiment was performed in triplicated wells. The survival index (%) was calculated by the absorbance of each condition normalized to mean absorbance of no mAb in rabbit serum as 100%. Bar graphs show mean ± SD from six values of two independent experiments. Two-way ANOVA followed by Tukey's multiple comparisons test was used for comparison, ****P < 0.0001