Fig. 5.

TIGIT blockade enhance cytotoxicity of NK cells. Purified NK cells were cultured with IL-12 for 72 h, and were co-cultured with CFSE-labelled K562 at effector to target (E:T) ratio 10:1 for 4 h under the isotype control Ab (IgG), anti-TIGIT neutralizing Ab, and anti-CD226 neutralizing Ab. The cytotoxicity of effector NK cells was determined by 7-AAD + CFSE labeled K562 cells. a Quantification of the frequency of 7AAD⁺ CFSE labeled K562 cells in the presence of anti-TIGIT Ab or anti-CD226 Ab blockade. b IFN-γ and CD107a expression by effector NK cells in the presence of anti-TIGIT Ab or anti-CD226 Ab blockade. HD, healthy donors. Each symbol represents one subject and the data are shown as the mean (± SEM). P values, one-way ANOVA followed by Dunnett’s multiple-comparisons test (a, b), *, P < 0.05; **, P < 0.01