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. 2022 May 31;9(1):62. doi: 10.1186/s40643-022-00547-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Diagram showing the metabolic pathway of succinic acid of engineered E. coli under anaerobic condition. The dissimilation metabolic pathway of methanol and formate and a new pathway of fixing CO2 were introduced. G6P glucose-6-phosphate, G3P 3-phosphate-glyceraldehyde, PEP phosphoenolpyruvic acid, OAA oxaloacetate, PYC pyruvate, Mdh methanol dehydrogenase, Fdh formate dehydrogenase, Pyc pyruvate carboxylase