Fig. 1.

Metformin inhibited the proliferation of NSCLC. A Metformin has a concentration-dependent inhibitory effect on NSCLC cells. B, C The volcano map and heatmap show the differential expressed genes. D qPCR showed the mRNA of PDL1 in A549 and H1299 significantly reduced after metformin treatment. E The DNA fragment pulled by the CEBPB antibody was the fragment of − 95 to  + 55 bp. F The PCR products were used for electrophoresis. Metformin concentration. NC: no metformin treatment. M1: 0.15 mmol/L (A549), 1 mmol/L (H1299). M2: 0.25 mmol/L (A549), 2 mmol/L (H1299)