Fig. 6.
Induction of cell death and clustering by fcɤR-mediated cross-linking of DARA. BCBL-1, GTO, TY-1 cells were pre-incubated for 15 min with varying concentrations of DARA followed by an overnight incubation in the presence of either 25 μg/ml of rabbit anti-human IgG F(ab’)2 fragments or goat anti-mouse IgG (H + L). A The percentage of cell viability was assessed by MTT assay. B DARA cross-linking-mediated clustering of BCBL-1 cells. Bright-field images of BCBL-1 after 18 h of incubation with a range of DARA concentration in the presence of 25 μg/ml Fc cross-linking secondary Ab