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. 2022 Nov 3;72(5):1089–1102. doi: 10.1007/s00262-022-03313-2

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© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

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Fig. 1 — Analysis of Treg chemokines in HNSCC cell lines. a Heat map of Treg chemokines in 33 HNSCC cell lines analyzed from the CCLE. b Statistical analysis of the top 3 most frequently expressed Treg chemokines in human HNSCC cell lines. Two-way ANOVA was used to assess statistical significance. c Categorization of HNSCC cell lines based on levels of CCL20 expression (red dots). None of the cell lines expressed the CCL20 receptor, CCR6 (blue dots). d qPCR analysis of transcript levels of CCL20 in MOC1 and MOC2 mouse HNSCCs. Expression was normalized to MOC1. e Analysis of secreted CCL20 in human and mouse cell lines. Error bars represent SEM of 4–5 independent experiments. Asterisks show statistical significance