Fig. 2.

Induction of CCL20 in response to high dose, but not low dose radiation and associated changes in the secretome. a Analysis of gene expression of CCL20 in human (Cal 27, SCC9) and murine (MOC1, MOC2) HNSCC cell lines after exposure to low (2 Gy) and high (10 Gy) doses of radiation. b Analysis of secreted CCL20 in MOC1 and MOC2 cell lines after radiation. Two-way ANOVA was performed to assess statistical significance. c Analysis of the pro-tumoral chemokines induced in response to 10 Gy radiation in MOC1 and MOC2 cells. d Analysis of anti-tumoral chemokines induced in response to 10 Gy radiation in MOC1 and MOC2 cells. Data for 10 Gy were normalized to 0 Gy. Dashed line represents the normalization value (equal to 1) for each chemokine. A value of 1 indicates no change in the respective chemokine. *P value < 0.05; **P value < 0.01