Fig. 5.

Effect of combination RT + anti-CCL20 on tumor growth in orthotopic HNSCCs. a Timeline of the experiment. b Validation of anti-CCL20 target specificity by assessment of plasma levels of CCL20 using ELISA. c Tumor volumes prior to irradiation in the IgG and anti-CCL20 groups (n = 16 per group). d Tumor growth assessment in the RT + anti-CCL20 group compared to IgG control, anti-CCL20 and RT + IgG. Two-way ANOVA was performed to assess significance between the groups on the last day of the experiment. e Analysis of CCL19, GM-CSF and IL-2 in plasma from anti-CCL20 and IgG treated mice. *P value < 0.05; **P value < 0.01