Fig. 3.

Effects of pH and temperature on surface-displayed InaPbN-CESH[L] enzymatic activity and stability. a Relative enzymatic activities in buffers with different pH values. The activities were measured after incubation at 4 °C for 5 h. The highest whole-cell enzymatic activity in Tris–HCl buffer (pH8.5) was set to 100%. b The long-term stability at 4 °C in Tris–HCl buffers with different pH values. c Trypsin accessibility assays of the InaPbN-CESH[L] displayed whole cells after incubation at 4 °C. Lanes 1–8, each pair of lanes are trypsin-untreated and treated whole cells with different lengths of incubation time. Lane M, standard protein molecular weight markers. d The long-term stability of the InaPbN-CESH[L] displayed whole cells incubated at different temperatures