Fig. 4.

Repeated use of the InaPbN-CESH[L] surface display system. The cells after protein expression were incubated at 4 °C for different days and then the whole-cell enzyme activities were repetitively assayed four times. After each enzymatic reaction, the cells were centrifuged and washed with the assay buffer, and then the whole-cell enzyme activities were immediately assayed again as repetitive usage