TABLE 3.
Marker rescue of wild-type viruses with plasmids carrying mutations in the UL5 gene
| Plasmida | Site-specific mutation | Codon change | Titer of rescued viruses (avg PFU/ml) |
|---|---|---|---|
| TP201 | UL5 wild type | None | 0 |
| TP202 | 355K-N | AAG-AAT | 5.0 × 104 |
| TP227 | 355K-V | AAG-GTA | 0 |
| TP205 | 355K-T | AAG-ACG | 1.0 × 103 |
| TP206 | 355K-L | AAG-TTG | 3.0 × 102 |
| TP213 | 355K-C | AAG-TGC | 4.0 × 10 |
| TP212 | 355K-Q | AAG-CAG | 3.0 × 102 |
| TP214 | 355K-S | AAG-AGC | 1.4 × 104 |
| TP215 | 355K-I | AAG-ATA | 3.0 × 102 |
| TP216 | 355K-M | AAG-ATG | 0 |
| TP217 | 355K-G | AAG-GGG | 0 |
| TP218 | 355K-R | AAG-AGA | 2.0 × 102 |
| TP220 | 355K-D | AAG-GAC | 0 |
| TP221 | 355K-E | AAG-GAA | 0 |
| TP222 | 355K-H | AAG-CAC | 3.0 × 103 |
| TP223 | 355K-P | AAG-CCA | 0 |
| TP224 | 355K-W | AAG-TGG | 1.3 × 102 |
| TP219 | 355K-Y | AAG-TAC | 0 |
| TP208 | 354M-C | ATG-TGC | 3.0 × 103 |
| TP231 | 354M-L | ATG-TTG | 0 |
| TP209 | 354M-A | ATG-GCC | 0 |
| TP210 | 354M-V | ATG-GTC | 0 |
a
Transfections were performed in triplicate with 50, 100, or 200 ng of plasmid DNA and 1 μg of rHSV-2LUC DNA.