FIGURE 5.
Production of influenza vaccine‐relevant virus strains using top cell clones in an ambr15 system. Cells were seeded into an ambr15 vessel and grown for 3 days (triangles: C59 and C113 in MDXK medium, diamond‐shape, gray: Xe.A in 4Cell medium). Subsequently, cells were two‐fold diluted in trypsin‐containing medium to about 2 × 106 cells/mL and infected with A(H1N1), A/(H3N2), B(Victoria), or B(Yamagata) at a MOI of 10−3. Left panel: Viable cell concentration (solid lines, full symbols) and cell viabilities (dashed lines, empty symbols), gray background: cell growth phase. Right panel: Infectious (solid lines, full symbols) and total (dashed lines, empty symbols) virus titers determined by HA and TCID50 assay, respectively.