TABLE 2.

Growth characteristics of monoclonal suspension MDCK cells.

Cell line/clone	VCCmax [106 cells/mL]	D [µm]	tD [h]	qGlc [fmol/(cell × h)]	qGln [fmol/(cell × h)]	qLac [fmol/(cell × h)]	qNH4 [fmol/(cell × h)]	Yglc/lac [−]	YNH4/gln [−]
C15‐1	3.8	15.5–17.5	31	−135	−48	240	49	0.6	1.0
C15‐2	4.2	15.5–17.2	37	−114	−38	189	40	0.6	1.0
C26	3.8	15.2–17.8	46	−178	−50	197	43	0.9	0.9
C59	8.8	14.8–16.5	26	−78	−26	102	15	0.8	0.6
C59‐p70	17.3	13.8–15.4	20	−66	−24	75	17	0.9	0.8
C86‐1	3.7	15.3–18.2	42	−143	−35	216	35	0.7	1.0
C86‐2	4.1	17.1–18.9	33	−130	−39	180	34	0.7	0.9
C113	4.2	15.9–18.9	47	−140	−44	174	41	0.8	0.9
C113‐p70	5.5	17.0–19.6	22	−217	−72	262	79	0.8	1.1
Xe.A	17.1	13.7–16.3	20	−54	−18	72	12	0.7	0.6

Note: Maximum viable cell concentrations (VCC_max), cell diameter (d), doubling time (t_D) for a growth period of 72 h for all cell clones and 96 h for reference Xe.A cells and C59‐p70 cells, respectively; consumption rates (qs) of the main metabolites glucose, glutamine, lactose and ammonium; negative values indicate uptake and positive values release (for profiles see Figure S1); Y represents the yield coefficient for the conversion of the respective metabolites determined from the absolute difference over time.