TABLE 3.
Summary of A(H1N1) production in different monoclonal suspension MDCK cell lines in ambr15 vessels and 1 L STR (MDXK medium).
| System | Cell line/clone | VCCmax [106 cells/mL] | HAmax [log10(HAU/100 µL)] | TCIDmax [109 TCID50/mL] | CSVYHA [virions/cell] | CSVYTCID [virions/cell] |
|---|---|---|---|---|---|---|
| ambr15 | C15‐2 | 3.6 ± 0.3 | 3.08 ± 0.08 | 1.1 ± 0.3 | 6977 | 321 |
| C59 | 3.2 ± 0.3 | 2.55 ± 0.16 | 0.4 ± 0.3 | 2277 | 135 | |
| C86‐1 | 2.5 ± 0.4 | 3.13 ± 0.17 | 0.6 ± 0.3 | 11,342 | 236 | |
| C86‐2 | 2.6 ± 0.4 | 3.08 ± 0.15 | 0.7 ± 0.3 | 9599 | 281 | |
| C113 | 3.1 ± 0.3 | 3.43 ± 0.03 | 0.9 ± 0.1 | 17,771 | 294 | |
| Xe.A | 4.0 ± 0.3 | 3.17 ± 0.06 | 1.0 ± 0.2 | 7694 | 258 | |
| 1 L STR | C59 | 4.93 | 2.97 | 1.0 | 3900 | 203 |
| C113 | 3.07 | 3.34 | 1.3 | 14,680 | 346 | |
| Xe.A | 6.46 | 3.31 | 1.8 | 6436 | 265 |
Note: Maximum viable cell concentration (VCCmax), maximum virus titers (HAmax and TCIDmax), and cell‐specific virus yields (HA‐based: CSVYHA, TCID50‐based: CSVYTCID). For the ambr15 system, mean and standard deviation of three vessels inoculated from same pre‐culture. STR results are from a single run. Xe.A: reference cultivated in 4Cell medium.