TABLE 4.
Production of different influenza vaccine‐relevant strains in C59, C113, and Xe.A cells in ambr15 vessels.
| Virus | Cell line/clone | VCCmax [106 cells/mL] | HAmax [log10(HAU/100 µL)] | TCIDmax [TCID50/mL] | CSVYHA [virions/cell] | CSVYTCID [virions/cell] |
|---|---|---|---|---|---|---|
| A(H1N1) | C59 | 4.3 | 2.92 | 5.6 × 108 | 3784 | 130 |
| C113 | 2.9 | 3.45 | 1.0 × 109 | 19,413 | 350 | |
| Xe.A | 7.8 | 3.45 | 2.4 × 109 | 7137 | 305 | |
| A(H3N2) | C59 | 4.3 | n.d. | 7.5 × 105 | n.d. | <1 |
| C113 | 2.7 | 2.37 | 1.3 × 106 | 1730 | <1 | |
| Xe.A | 5.1 | 1.32 | 1.3 × 106 | 80 | <1 | |
| B(Victoria) | C59 | 2.8 | 2.98 | 5.6 × 106 | 6841 | 2 |
| C113 | 4.5 | 3.28 | 7.5 × 107 | 8398 | 17 | |
| Xe.A | 6.8 | 1.72 | 3.2 × 106 | 152 | <1 | |
| B(Yamagata) | C59 | 4.3 | 3.05 | 1.8 × 108 | 5140 | 41 |
| C113 | 2.2 | 3.45 | 1.3 × 108 | 25,353 | 61 | |
| Xe.A | 6.5 | 3.45 | 1.8 × 108 | 8542 | 27 |
Note: Maximum viable cell concentration (VCCmax), maximum titers (HAmax and TCIDmax), and cell‐specific virus yields (HA assay‐based: CSVYHA, TCID50 assay‐based: CSVYTCID). n.d.: not detectable, HA value below limit of detection (0.45 log10(HAU/100 µL)). All results depicted are from a single cultivation in ambr15 vessels.