Figure 5.

Heatmaps showing changes in the constitutive expression of stress-involved genes revealed by RT-qPCR in (A, E) hypothalamic nucleus preopticus (NPO), (B, F) pituitary gland (PIT), (C, G) head kidney (HK), and (D, H) gills of uninfected (Ctr) and CEV-infected (CEV) fish from three groups of carp: koi (Koi), salt-treated koi (Koi NaCl), and Amur sazan (Amur) kept at 12°C (A–D) and 18°C (E–H) (6 days post exposure). Asterisks indicate statistically significant differences between uninfected and CEV-infected fish within the strain/group (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001). Different lowercase letters (e.g., a vs. b) indicate statistically significant differences at p ≤ 0.05 between the strains/groups of uninfected fish. Different capital letters (e.g., A vs. B) indicate statistically significant differences at p ≤ 0.05 between the groups of CEV-infected fish. Statistical analysis was performed using two-way ANOVA with subsequent pairwise multiple comparisons using the Holm-Sidak test. The data are the mean of n = 8 fish.