Figure 7.

Heatmaps showing changes in gene expression of immune-related genes revealed by nanoscale RT-qPCR in gills of uninfected (Ctr) and CEV-infected (CEV) fish from three groups of carp: koi (Koi), salt-treated koi (Koi NaCl), and Amur sazan (Amur) kept at 18°C (6 days post exposure). Asterisks indicate statistically significant differences between uninfected and CEV-infected within the strain/group (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001). Different lowercase letters (e.g., a vs. b) indicate statistically significant differences at p ≤ 0.05 between the strains/groups of uninfected fish. Different capital letters (e.g., A vs. B) indicate statistically significant differences at p ≤ 0.05 between the strains/groups of CEV-infected fish. Statistical analysis was performed using two-way ANOVA, with subsequent pairwise multiple comparisons using the Holm–Sidak test. For each group, n = 7–8 fish are presented. One square equals data from one individual fish.